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Part:BBa_K4388012

Designed by: Tomas Lopes   Group: iGEM22_KCL_UK   (2022-10-08)


Linker Sequence Replacing Terminator

This linker sequence can be used to replace terminators in a synthetic operon system, where all genes contain this downstream linker to allow transcription to continue, except for the last gene where a terminator is placed downstream thus terminating transcription and resulting in a single mRNA for the operon. Therefore this sequence is useful in assembly systems that require 4 modules for each transcriptional unit (promoter, RBS, gene, terminator) and a synthetic operon system is desired.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None